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Abstract
BackgroundHundreds of HSP40s derived from various species have been identified, of which several proteins are involved in spermatogenesis. DNAJB13 is a type II HSP40/DnaJ protein. In a previous study, we cloned mouse Dnajb13, which is up-regulated in cryptorchidism. To date, however, little is known about the localization and functions of DNAJB13 during spermatogenesis. This study intends to identify the expression pattern of DNAJB13 during mammalian spermatogenesis.ResultsRT-PCR and western blot revealed that the Dnajb13 gene and DNAJB13 protein were expressed not only in the testis but also in several other ciliated cell-containing tissues like the trachea, lung and oviduct. Quantitative PCR showed that the expression of Dnajb13 mRNA in testis was detectable as early as postnatal week 1, and sharply increased from postnatal week 3. Western blotting and immunohistochemistry determined that the DNAJB13 protein, which was located in the cytoplasm of spermatids and the sperm flagellum, was detectable from postnatal week 4.ConclusionsBased on the spatiotemporal expression observed in the cytoplasm of spermatids and sperm flagella, we suggest that DNAJB13 participates in spermiogenesis and the motility of mature spermatozoa.
Highlights
Hundreds of HSP40s derived from various species have been identified, of which several proteins are involved in spermatogenesis
The results showed that the Dnajb13 gene was extremely strongly expressed in adult testis, lung, oviduct and trachea (Figure 1A)
The results showed that DNAJB13 was expressed in adult testis, lung, oviduct, and trachea (Figure 1B), being consistent with the reverse transcription polymerase chain reaction (RT-PCR) results
Summary
Hundreds of HSP40s derived from various species have been identified, of which several proteins are involved in spermatogenesis. Spermatogenesis is a complex biological process involving a number of cellular events including mitosis, meiosis, cell migration, apoptosis and differentiation, which enable the germ cells to undergo several developmental stages, from spermatogonia to primary and secondary spermatocytes, round spermatids and eventually spermatozoa. It takes approximately 35 days in mice. Hundreds of HSP40s derived from various species have been identified, of which several proteins are involved in spermatogenesis [1]. The Dnajb (Msj-1) gene encodes a DnaJ protein highly
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