Abstract
Topology of the plasmid pGT5 from the hyperthermophilic archaeon Pyrococcus abyssi has been studied as a function of growth phase and temperature. Plasmids isolated from cells in mid-exponential or stationary phases had similar linking number (Lk), whilst plasmids isolated from cells grown at 95 °C had a higher Lk than those isolated from cells grown at 80 °C. A significant reduction of Lk was observed when cells cultivated at 95 °C were slowly cooled at room temperature.
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