Abstract
The present study deals with ultraviolet-light (uv) sensitivity and DNA repair as well as semiconservative DNA synthesis in Cockayne's syndrome (CS) cells in vitro. Homozygous CS cell lines were found to be more uv sensitive in colony-forming ability than normal human fibroblast cell lines. There was no difference in either excision repair or postreplication repair following uv irradiation when comparing CS cells with normal cells. Semiconservative DNA synthesis of both CS cells and normal cells was depressed to the same extent by uv irradiation. The subsequent recovery of CS cell lines, however, was reduced. This reduction in recovery appears to be caused by inhibition of replicon initiation following uv irradiation. These processes in the heterozygous CS cell line were indistinguishable from those of normal cells. These findings support the hypothesis that uv-induced chromatin alteration in no relation to well-known DNA repair processes persists longer in CS cells than in normal cells. This condition results in inhibition of replicon initiation.
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