Abstract
The status of DNA methylation, as measured by m 5C 2 2 Abbreviations used are: m 5C, 5-methylcytosine; HPLC, high pressure liquid chromatography; HTC, hepatoma tissue culture; TAT, tyrosine aminotransferase content of nuclear DNA, was examined during corticosteroid mediated TAT induction in rat liver and in dividing and nondividing HTC cells. The m 5C content, determined by HPLC, was not significantly altered in HTC cells during TAT induction whether the cells were in the logarithmic or stationary growth phase. In the liver of adrenalectomized rats where the range of corticosteroid effects is greater than in HTC cells, a small but significant decline in genomic levels of m 5C was detected between 1 to 8 hr post-induction. The alterations in DNA methylation did not fluctuate during induction by more than 8% in the liver or 7.5% in HTC cells. These results demonstrate that no gross change or elevation in m 5C content is detected in two, different, hormonally responsive hepatocellular systems during gene activation.
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