DNA identification of Zijingpi's adulterant species Schisandra sphenanthera based on NCBI nucleotide database analysis

Abstract

To provide basis for quality control of Zijingpi, DNA identification was used based on NCBI nucleotide database analysis. Firstly, total DNA of Zijingpi was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and PCR products was directly sequenced after purification. Finally, ITS sequence similarity and phylogenetic tree were used for identification. The ITS sequence information of the mainstream commercial drugs of Zijingpi was obtained. It is firstly reported that the mainstream commercial drugs of Zijingpi was the bark of Schisandra sphenanthera.