STAR protocols | VOL. 3
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DNA fiber combing protocol using in-house reagents and coverslips to analyze replication fork dynamics inmammalian cells.

Publication Date Jun 17, 2022

Abstract

DNA fiber combing is a versatile technique that provides insight into replication fork dynamics at single-molecule resolution. DNA fibers are bound to silanized coverslips and combed, which straightens and aligns the fibers along a single axis. Here, we present a DNA fiber combing protocol that does not use commercial kits; we detail the steps to prepare all materials, reagents, and silanized coverslips. We describe the use of DLD-1 cells, but the protocol is amenable to other cell types.

Concepts

Replication Fork Dynamics In-house Reagents Single-molecule Resolution DNA Fiber Single Axis Commercial Kits Cell Types Dynamics In Mammalian Cells Fork Dynamics DLD-1 Cells

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No potential conflict of interest was reported by the authors. The conception and design of the study, acquisition of data, analysis and interpretatio...

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