Abstract
Taxonomic recognition of tiny false spider mites using morphological features is not always easy due to their small size, especially in their immature stage. In the past decade or so, polymerase chain reaction has been introduced into molecular systematics. However, a major problem with using molecular markers in mite systematics is that sometimes not enough DNA template can be acquired from a single individual for DNA amplification. To solve this problem, we developed a nested PCR of individual false spider mites with an adult body size of ca. 300 μm, for DNA amplification. A dilution of up to 10^5 of the DNA from a single egg, a larva, a nymph or an adult mite contains enough template for the amplification of the target DNA, i.e., 28S ribosomal DNA.
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