Abstract
The effects of monensin, an activator of Na +/H + exchanger (NHE), on capacitative Ca 2+ entry (CCE) were investigated using A7r5 cells. Capacitative Ca 2+ entry was induced by elevation of extracellular Ca 2+ concentrations of A7r5 cells in which stored Ca 2+ had been depleted by previous administration of thapsigargin. Capacitative Ca 2+ entry was abolished by pretreatment of the cells with SKF-96365 (1-[β-(3-[4-methoxyphenyl]propoxy)-4-methoxyphenethyl]-1 H-imidazole hydrochloride) but was not affected by pretreatment with verapamil. Monensin significantly increased capacitative Ca 2+ entry. On the other hand, 5-hydroxytryptamine-induced inositol monophosphate accumulation and subsequent intracellular Ca 2+ release from its stores were significantly inhibited by monensin, while thapsigargin-induced Ca 2+ release was not affected by monensin. These results suggest that monensin has diverse actions on capacitative Ca 2+ entry and agonist-induced release of stored Ca 2+ in vascular smooth muscle cells.
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