Diuretic Activity of <i>Hibiscus sabdariffa</i> L. in Wistar Rats

Abstract

The diuretic effect of aqueous extract of Hibiscus sabdariffa has always been noted by the early botanists who studied the plant. On the other hand, very little scientific work to confirm this state of affairs has been done. In this pharmacological study, we provide scientific evidence to previous observations. Using an electrolyte analyzer to evaluate urinary volume and ions and appropriate experimental protocols on wistar strain rats, we have shown that: The aqueous extract of Hibiscus sabdariffa (AEHS) administered at doses of 200, 500 and 700 mg / kg BW induces a dose-dependent and significant increase in urinary excretion in rats. This increase was 12.75 ± 1.971 mL / kg, 23.20 ± 2.082 mL / kg and 29.42 ± 2.097 mL / kg BW, respectively is after 24 hours. That is a respective increase of 55.5, 182 and 333% compared to the control. The aqueous extract of Hibiscus sabdariffa (AEHS) uses at the dose of 700 mg / kg BW results in a reduction of the first urination time by 26.30% compared to the control. The determination of electrolytes in rats treated with 700 mg / kg BW after 24 hours showed high concentrations of sodium, potassium and chlorine in the urine. These different concentrations are for sodium of 72.51%, potassium of 76.54% and 58.81% for chlorine relative to the concentration of the controls. Compared to AEHS, hydrochlorothiazide at 25 mg / kg BW results in urinary excretion of 80.79% sodium, 85.30% potassium and 75.97% chlorine. Similarly with furosemide uses at 20 mg / kg BW), the concentrations are 86% sodium, 79% potassium and 71.58% chlorine. Compared with control rats, the Na + / K + ratio did not vary significantly in the treated animals. AEHS is therefore a diuretic substance like hydrochlorothiazide and furosemide.