Abstract
Filipin, a membrane beta-hydroxysterol probe, and polymyxin B (PXB), a probe for anionic lipids, were used to study human sperm plasma membrane (PM) with particular reference to changes induced by capacitation in vitro. In washed but noncapacitated spermatozoa the density of filipin/sterol complexes (FSC) was uniformly high in the PM overlying the acrosome, without any differences between its anterior and equatorial regions. The postacrosomal region was poor in FSC. The exclusion of FSC from small areas of the PM covering the two acrosomal regions and reduction of their number in the postacrosomal region were the main changes induced by sperm capacitation. Unlike filipin, PXB produced a capacitation-independent reaction pattern characterized by a high reactivity of the PM covering the anterior portion of the acrosome, probably conferring to this region its potential fusigenicity temporarily antagonized by the elevated sterol content. Only an exceptional and slight PXB binding was seen in the nonfusigenic equatorial and postacrosomal regions.
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