Abstract
A steady-state fluorescence quenching study has been undertaken to estimate aggregation numbers, N, and the average occupancy number ( n ¯ ) of quencher per micelle and the water pool size, r w, of nonionic surfactant Igepal (polyoxyethylene nonyl phenol), carbon tetrachloride and water reverse micelles at a different [water]/[Igepal] ratio. For the purpose, Safranine T (3,7-diamino-2,8-dimethyl-5-phenyl phenazinium chloride) has been employed as fluorescent probe and iodide, bromide and azide ions as static quencher. The fluorescence quenching data has been exploited to determine the nature and properties of water in the core of reverse micelles (RM), size of the water pool, association of quencher molecule with the RM, the localization of the probe and the distribution of quencher molecule in the quenching sphere. The demarcation of reverse micelles and microemulsion has also been determined.
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