Abstract
The distribution of nerve growth factor (NGF) was studied after topical application ofNGF solution or NGF Minipellet into the caudate nucleus of cat brains. Each aliquot of NGF solution or a piece of NGF Minipellet contains 200 µ1g of mouse 2.5S β-NGF. The concentrations of NGF In vanous areas of the braIn were determined by enzyme-linked immunosolvent assay (ELISA). The tissue concentrations of NGF were very high at 6h after injection of NGF solution (124.0±2.46 to 2144.0± 16.03 ngg-1), but quickly decreased on day 1. The tissue concentrations of NGF were low at 6 h after Implantation of NGF Minipelletl but were considerably increased on day 1 (11.22 ± 3.36 to 72.04 ± 20.45 ngg-1), and slowly decreased during the following 6 to 14 days. The present study demonstrates the temporal and spatIal profiles ofNGF distribution in the brain of middle-sized animals after topical application. Injection of NGF solution resulted in abruptl but very transientl elevation of tissue NGF. Implantation of NGF Minipellet maintained the tissue NGF at biologically effective levels for 6 to 14 days. [Neural Res 1998, 20: 116–120)
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