Abstract
BackgroundMacrophages and plasma cells play a key role in the regulation of innate and adaptive immunity. The aim of this study was to assess the presence of these cells in apical periodontitis and their distribution comparing with clinical and image data.Material and MethodsThirty-three lesions were selected and divided in two groups (17 periapical cysts and 16 periapical granulomas). Immunoreactions using anti-CD68 and anti-CD138 antibodies were carried out; image analysis was performed with an optical microscope and 5 high-power fields from each slide were evaluated leading to an average score of immunoexpression. This mean score was compared between the two groups and correlated with the clinical and image data.ResultsThere was no statistically significant difference (p >0.05) for the mean average score of CD68+ macrophages and CD138+ plasma cells when comparing the two groups (cysts x granulomas) and the specimens included in each specific group. No statistically significant differences (p >0.05) were also observed when comparing the average scores with clinical and image data.ConclusionsThe presence of CD68+ macrophages and CD138+ plasma cells was similar in periapical cysts and granulomas and the presence of these cells did not correlate with clinical and image data from both groups. Key words:Macrophages, plasma cells, apical periodontitis, periapical granuloma, periapical cyst.
Highlights
Apical periodontitis (AP) is the main response of periradicular tissues to a variety of stimuli that cause pulpal and periradicular damage [1]
There was no statistically significant difference when comparing the mean score of CD68+ macrophages in cysts (0.83 ± 0.63) and granulomas (0.81 ± 0.39) (p>0.05) (Fig. 1A)
The present results support that macrophages and plasma cells are common components of the inflammatory infiltrate in both periapical cysts and granulomas
Summary
Apical periodontitis (AP) is the main response of periradicular tissues to a variety of stimuli that cause pulpal and periradicular damage [1]. The aim of this study was to assess the presence of macrophages and plasma cells in periapical granulomas and cysts, comparing the results with clinical and image data. Immunoreactions using anti-CD68 and anti-CD138 antibodies were carried out; image analysis was performed with an optical microscope and 5 high-power fields from each slide were evaluated leading to an average score of immunoexpression. This mean score was compared between the two groups and correlated with the clinical and image data. Results: There was no statistically significant difference (p>0.05) for the mean average score of CD68+ macrophages and CD138+ plasma cells when comparing the two groups (cysts x granulomas) and the specimens included in each specific group. Conclusions: The presence of CD68+ macrophages and CD138+ plasma cells was similar in periapical cysts and granulomas and the presence of these cells did not correlate with clinical and image data from both groups
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