Abstract
BackgroundIndividual differences between human embryonic stem cell (hESC) lines are poorly understood. Here, we describe the derivation of five hESC lines (called FES 21, 22, 29, 30 and 61) from frozen-thawed human embryos and compare their individual differentiation characteristic.ResultsThe cell lines were cultured either on human or mouse feeder cells. The cells grew significantly faster and could be passaged enzymatically only on mouse feeders. However, this was found to lead to chromosomal instability after prolonged culture. All hESC lines expressed the established markers of pluripotent cells as well as several primordial germ cell (PGC) marker genes in a uniform manner. However, the cell lines showed distinct features in their spontaneous differentiation patterns. The embryoid body (EB) formation frequency of FES 30 cell line was significantly lower than that of other lines and cells within the EBs differentiated less readily. Likewise, teratomas derived from FES 30 cells were constantly cystic and showed only minor solid tissue formation with a monotonous differentiation pattern as compared with the other lines.ConclusionhESC lines may differ substantially in their differentiation properties although they appear similar in the undifferentiated state.
Highlights
Individual differences between human embryonic stem cell lines are poorly understood
Conclusion: human embryonic stem cell (hESC) lines may differ substantially in their differentiation properties they appear similar in the undifferentiated state
Of the 83 blastocysts, 70 inner cell mass (ICM) were successfully isolated onto human foreskin fibroblast (HFF) or mouse embryonic fibroblast (MEF) feeder cells
Summary
Individual differences between human embryonic stem cell (hESC) lines are poorly understood. Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of the mammalian blastocyst. While there are plenty of studies regarding differentiation of hESC into a variety of cell types, only a few studies address the differences between individual cell lines and they are mainly focused on transcriptional profiling. These studies have indicated that the mRNA expression patterns do vary to some extent between the hESC lines, biological significance of the variation has not been studied in detail [5,6,7,8,9]
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