Abstract
Key messageTwo QTL with pleiotropic effects on plant height and spike length linked in coupling phase on chromosome 2DS were dissected, and diagnostic marker for each QTL was developed.Plant height (PHT) is a crucial trait related to plant architecture and yield potential, and dissection of its underlying genetic basis would help to improve the efficiency of designed breeding in wheat. Here, two quantitative trait loci (QTL) linked in coupling phase on the short arm of chromosome 2D with pleiotropic effects on PHT and spike length, QPht/Sl.cau-2D.1 and QPht/Sl.cau-2D.2, were separated and characterized. QPht/Sl.cau-2D.1 is a novel QTL located between SNP makers BS00022234_51 and BobWhite_rep_c63957_1472. QPht/Sl.cau-2D.2 is mapped between two SSR markers, SSR-2062 and Xgwm484, which are located on the same genomic interval as Rht8. Moreover, the diagnostic marker tightly linked with each QTL was developed for the haplotype analysis using diverse panels of wheat accessions. The frequency of the height-reduced allele of QPht/Sl.cau-2D.1 is much lower than that of QPht/Sl.cau-2D.2, suggesting that this novel QTL may be an attractive target for genetic improvement. Consistent with a previous study of Rht8, a significant difference in cell length was observed between the NILs of QPht/Sl.cau-2D.2. By contrast, there was no difference in cell length between NILs of QPht/Sl.cau-2D.1, indicating that the underlying molecular mechanism for these two QTL may be different. Collectively, these data provide a new example of QTL dissection, and the developed diagnostic markers will be useful in marker-assisted pyramiding of QPht/Sl.cau-2D.1 and/or QPht/Sl.cau-2D.2 with the other genes in wheat breeding.
Highlights
Wheat (Triticum aestivum L.) is an important food crop worldwide, providing calories and proteins consumed by humankind (Fischer et al 2014; Shiferaw et al 2013)
These data provide a new example of quantitative trait loci (QTL) dissection, and the developed diagnostic markers will be useful in marker-assisted pyramiding of QPht/Sl.cau-2D.1 and/or QPht/Sl.cau-2D.2 with the other genes in wheat breeding
All increasing alleles for Plant height (PHT) and spike length (SL) came from Jing 411 (J411) (Table 1)
Summary
Wheat (Triticum aestivum L.) is an important food crop worldwide, providing calories and proteins consumed by humankind (Fischer et al 2014; Shiferaw et al 2013). 24 genes influencing PHT have been identified and designated reduced height genes in wheat (McIntosh et al 2017) Of these 24 major genes, Rht-B1b and Rht-D1b, which are located on chromosomes 4B and 4D, respectively (Pearce et al 2011), were cloned and most widely used in wheat breeding (Borner et al 2002; Cadalen et al 1998). RhtB1b and Rht-D1b belonged to the group of dwarfing genes that is insensitive to gibberellic acid (Pearce et al 2011) The characterization of these two genes has enhanced our knowledge about PHT determination in wheat, and functional markers developed for them have been used in wheat breeding (Akman and Bruckner 2012; Borrell et al 1991; Tang et al 2009). Haplotype distribution of QPht.cau-2D.1 and QPht. cau-2D.2 in diverse panels of wheat accessions was investigated
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