Abstract
BackgroundIn resource-limited countries, ABO, HLA, MNS, Kells, and hemoglobin electrophoresis are classic tests for the resolution of paternity disputes due to their affordable cost. The limitations of these tests in cases of disputed paternity require the use of Short Tandem Repeats (STR) for their certification. This study aimed to determine the biological fathers of children using ABO-rhesus/hemoglobin electrophoresis and STR assays in Burkina Faso, West Africa. ResultsOf the fourteen trios studied, the ABO-rhesus/hemoglobin electrophoresis analysis revealed ten probable inclusion cases, three exclusion cases, and one undetermined paternity. DNA STR analysis found five inclusions of paternity out of the ten probable inclusions with ABO-rhesus/hemoglobin electrophoresis assay versus nine exclusions of paternity. ConclusionThis study showed that the implementation of the analysis of short tandem repeat is required to resolve increasing disputed filiation cases in Burkina Faso.
Highlights
In resource-limited countries, ABO, HLA, MNS, Kells, and hemoglobin electrophoresis are classic tests for the resolution of paternity disputes due to their affordable cost
Despite the technical limitations with a high risk of misidentification of the biological father, ABO-rhesus/Hb electrophoresis has been used for a long time to resolve paternity disputed cases in Burkina Faso, because of their affordability and the absence of Short Tandem Repeats (STR) assays
The paternity index ranged from 0 to 37, 072, 170, and 900 and the highest probability of paternity (POP) was 99.99999999997% found in trio 3 (Table 3)
Summary
In resource-limited countries, ABO, HLA, MNS, Kells, and hemoglobin electrophoresis are classic tests for the resolution of paternity disputes due to their affordable cost. Millogo et al Journal of Genetic Engineering and Biotechnology (2021) 19:130 limited when there is correspondence between the Hb genotype of the alleged father–mother and child Taking these limitations into account, in the context of paternity research, it is necessary to combine several systems [ABO, rhesus, HLA, MNS, Kell, serum systems...] or to use other more efficient systems such as microsatellite genetic analysis, or “short tandem repeats” (STR) [7, 8]. The present pioneer study aimed to determine the biological fathers of children using the old ABO-rhesus/Hb electrophoresis method and STR assays for the first time in Burkina Faso, West Africa
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