Abstract
A displacement chromatographic method has been developed for the preparative separation of the enantiomers of 5,10-dideazatetrahydrofolic acid on a β-cyclodextrin silica column. The retention behavior of the chiral solute was studied in detail to find the separation conditions which provide the largest chiral selectivity values: the κ′ vs. acetonitrile modifier concentration, the κ′ vs. pH, the κ′ vs. citrate buffer concentration and the κ′ vs. 1/ T relationships; also, the α vs. acetonitrile concentration, the α vs. pH, the α vs. citrate buffer concentration and the log α vs. 1/ T relationships have been determined. Cetyltrimethylammonium bromide, more retained and more strongly adsorbed than the chiral solute, was selected as displacer for the separation. With an α value of 1.14, good preparative chiral separations were observed both in the displacement mode and in the overloaded elution mode.
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