Abstract
High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy is a useful tool for investigating the metabolism of various cancers. Basal cell carcinoma (BCC) is the most common skin cancer. However, to our knowledge, data on metabolic profiling of BCC have not been reported in the literature. The objective of the present study was to investigate the metabolic profiling of cutaneous BCC using HR-MAS 1H NMR spectroscopy. HR-MAS 1H NMR spectroscopy was used to analyze the metabolite profile and metabolite intensity of histopathologically confirmed BCC tissues and normal skin tissue (NST) samples. The metabolic intensity normalized to the total spectral intensities in BCC and NST was compared, and multivariate analysis was performed with orthogonal partial least-squares discriminant analysis (OPLS-DA). P values < 0.05 were considered statistically significant. Univariate analysis revealed 9 metabolites that showed statistically significant difference between BCC and NST. In multivariate analysis, the OPLS-DA models built with the HR-MAS NMR metabolic profiles revealed a clear separation of BCC from NST. The receiver operating characteristic curve generated from the results revealed an excellent discrimination of BCC from NST with an area under the curve (AUC) value of 0.961. The present study demonstrated that the metabolite profile and metabolite intensity differ between BCC and NST, and that HR-MAS 1H NMR spectroscopy can be a valuable tool in the diagnosis of BCC.
Highlights
Basal cell carcinoma (BCC), first described by Jacob in 1827, is the most common malignant neoplasm in humans [1]
normal skin tissue (NST) samples were acquired from 10 BCC patients; additional five normal tissues were collected from patients with other cancers who underwent surgical treatment
We have applied high-resolution magic angle spinning (HR-magic angle spinning (MAS)) 1H nuclear magnetic resonance (NMR) spectroscopy combined with biostatistical methods to explore the metabolic profiling of BCC
Summary
Basal cell carcinoma (BCC), first described by Jacob in 1827, is the most common malignant neoplasm in humans [1]. BCC has become an important public health problem and is a significant burden to the PLOS ONE | DOI:10.1371/journal.pone.0150328. Analysis of BCC Using 1H NMR Spectroscopy national health care service [3]. This increasing incidence is likely due to increased surveillance and a combination of multiple risk factors, which include increased sun exposure, ultraviolet or ionizing radiation, genetic defects, and immunosuppression. BCC arises from non-keratinizing cells in the basal layer of the epidermis. BCC grows slowly and rarely metastasizes, it can invade the surrounding tissues and cause local tissue destruction, functional impairment, and cosmetic disfiguration. Early diagnosis and treatment are crucial for a favorable prognosis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
