Abstract
Background In our previous studies, we discovered a series of chalcone-based phytopathogenic fungus inhibitors. However, knowledge of their effects, detailed targets and molecular mechanisms in Magnaporthe oryzae (M. oryzae) remained limited.MethodsTo explore the expression and function of differentially expressed genes in M. oryzae after treatment with compound C1, we analyzed the expression profile of mRNAs using a microarray analysis and GO, KEGG and WGCNA analysis, followed by qRT-PCR and Western blots to validate our findings.ResultsA total of 1013 up-regulated and 995 down-regulated mRNAs were differentially expressed after M. oryzae was treated with C1 compared to those of the control samples. Among these, cytochrome P450, glycylpeptide N-myristoyltransferase (NMT) and peroxisomal membrane protein 4 were identified as the most significant DEGs and were validated by experiments.ConclusionIn conclusion, our study suggests that the combination of transcriptomic microarray, bioinformatics analysis and weighted gene co-expression networks can be used to predict potential therapeutic targets and to map the pathways regulated by small molecular natural product-like drugs.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-3385-9) contains supplementary material, which is available to authorized users.
Highlights
In our previous studies, we discovered a series of chalcone-based phytopathogenic fungus inhibitors
In vitro antifungal assay The antifungal activity of compound C1 against B. maydis, S. scleotiorum, M. orzae, G. zeae and B. cintrea was investigated by measuring the inhibitory effects of radial growth in a petri dish with agar medium
The results demonstrate that C1 showed variable degrees of antifungal activity against the tested phytopathogen fungi
Summary
We discovered a series of chalcone-based phytopathogenic fungus inhibitors. Knowledge of their effects, detailed targets and molecular mechanisms in Magnaporthe oryzae (M. oryzae) remained limited. M. oryzae has gradually become resistant to existing fungicides; it is essential to discover novel, environmentally friendly compounds with high antifungal activity and clear molecular mechanisms of action (Xu et al 2015; Dong et al 2015; Wang et al 2014; Chen et al 2013). Past research has detailed the antifungal activities, the structure–activity relationships (SARs) and the inhibitory capacity of the fungal cell wall synthesis pathway for a series of chalcone derivatives (Lopez et al 2001, 2006, 2011; Svetaz et al 2004, 2007; Boeck et al 2005).
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