Abstract
Abstract. Knowledge about structural variations in genes and proteins relevant for fat traits is very important information to improve selection of breeding lines and preserve genetic variability in pig industry. The classical proteomics is a useful tool to separate and measure differentially expressed proteins in fat tissue of phenotypic different individual pigs. Single nucleotide polymorphisms (SNP) in the swine adipocyte fatty-acid binding protein 3 (FABP3) and leptin gene (LEP) were related to adipocyte accumulation and associated with meat quality in pigs (NECHTELBERGER et al. 2001, KULIG et al. 2001, KMIEC et al. 2003).
Highlights
Knowledge about structural variations in genes and proteins relevant for fat traits is very important information to improve selection of breeding lines and preserve genetic variability in pig industry
Isoelectric focusing (IEF) with 24 cm of IPG strips with 8 000 v/ h and 12 % of SDS-PAGE gels stained with Commassie G-250 were used
After characterization and identification of differentially expressed spots between growth stages, 2 primers were designed from known nucleotide sequences of the corresponding genes fatty-acid binding protein 3 (FABP3) (AJ416019) and leptin gene (LEP) (NM213840)
Summary
Knowledge about structural variations in genes and proteins relevant for fat traits is very important information to improve selection of breeding lines and preserve genetic variability in pig industry. A total of 40 commercial pigs (Landrace × Duroc × Yorkshire) in 2 growth stages (150 and 210 days) were used from Swine Production Division at National Institute of Animal Science (NIAS). After characterization and identification of differentially expressed spots between growth stages, 2 primers were designed from known nucleotide sequences of the corresponding genes FABP3 (AJ416019) and LEP (NM213840).
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