Abstract
ABSTRACT Direct rooting was induced in Withania somnifera leaf segments using an IBA dip treatment. The segments dipped in IBA formed roots along the midrib region of the abaxial surface when placed on Murashige and Skoog's (MS) basal medium containing no plant growth regulators. The length of the dip treatments (10, 20 and 30 min) and strength of the MS media (¼, ½, and full-strength) treatments had no apparent effect on rooting, although maximum rooting (85.3 percent of the cultures) occurred when the leaf segments were placed on ½-strength MS medium after a dip treatment with 100 mg/liter IBA solution for 20 min. The average number and length of roots were 32.3 per culture and 5.6 cm, respectively. Only 20 percent of the cultures produced roots if explants were grown on full-strength MS medium supplemented with IBA.
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