Abstract
Consolidated bioprocessing (CBP), which integrates enzyme production, saccharification and fermentation into a one-step process, is a promising strategy for cost-effective ethanol production from starchy biomass. To gain insights into starch-based ethanol production using CBP, an extensive screening was undertaken to identify naturally occurring yeasts that produce ethanol without the addition of any amylases. Three yeast strains were capable of producing a significant amount of ethanol. Quantitative assays revealed that Scheffersomyces shehatae JCM 18690 was the strain showing the highest ethanol production ability. This strain was able to utilize starch directly, and the ethanol concentration reached 9.21 g/L. We attribute the ethanol-producing ability of this strain to the high levels of glucoamylase activity, fermentation potential and ethanol stress tolerance. This study strongly suggests the possibility of starch-based ethanol production by consolidated bioprocessing using natural yeasts such as S. shehatae JCM 18690.
Highlights
Consolidated bioprocessing (CBP), which integrates enzyme production, saccharification and fermentation into a one-step process, is a promising strategy for cost-effective ethanol production from starchy biomass
This study strongly suggests the possibility of starch-based ethanol production by consolidated bioprocessing using natural yeasts such as S. shehatae JCM 18690
We examined the starch-assimilating ability of yeast strains isolated from soil samples in the Kyoto area of Japan
Summary
Direct ethanol production from starch using a natural isolate, Scheffersomyces shehatae: Toward consolidated bioprocessing. Consolidated bioprocessing (CBP), which integrates enzyme production, saccharification and fermentation into a one-step process, is a promising strategy for cost-effective ethanol production from starchy biomass. It has been well established that bioethanol can be produced by yeasts, such as Saccharomyces cerevisiae, Scheffersomyces stipitis, and Kluyveromyces marxianus[9,10,11] These strains assimilate glucose and other monosaccharides derived from enzymatic hydrolysis of starch. To gain insights into ethanol production from starch through CBP, a comprehensive screening was performed for natural yeast strains that could produce high levels of ethanol from starch by a one-step CBP www.nature.com/scientificreports conversion without the need for addition of enzymes. We provide the possible mechanisms for the direct production of ethanol from starch
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