Abstract
Direct electrochemistry of hemoglobin (Hb) immobilized on the PSS/SWNTs film modified Au electrode was studied. The immobilized Hb displayed a couple of stable and well-defined redox peaks with the formal potential ( E 0′) of about 0.117 V (versus SCE) in a 0.1 M phosphate buffer solution (PBS) of pH 7.0. The dependence of E 0′ on the solution pH indicated that the direct electron transfer reaction of Hb was that the electrons transfer was accompanied by an equal number of protons in reaction process. Both single-wall carbon nanotubes (SWNTs) and poly(styrene sulfonic acid) sodium salt (PSS) could accelerate the electron transfer between Hb and the electrode. Using the PSS/Hb/SWNTs film modified Au electrode, the interaction between Hb and ribavirin was studied.
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