Abstract
Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity
Highlights
D IRECT electron transfer between redox proteins and enzymes with the electrode is of great significance for the understanding the electron transfer process in biological system.[1]
Scanning electron microscopy (SEM) image of the synthesized nickel oxide (NiO) nanomaterial was recorded with the result shown in Figure 1A, which exhibited a 3D flower-like microstructure with the average size of 2– 3 μm
A pair of well-defined redox peaks appeared on CTS/NiO-horseradish peroxidase (HRP)/carbon ionic liquid electrode (CILE), which indicated that direct electron transfer of HRP was realized
Summary
D IRECT electron transfer between redox proteins and enzymes with the electrode is of great significance for the understanding the electron transfer process in biological system.[1]. Among nanomaterials used nanosized metal oxide can act as suitable matrix for the immobilization of redox proteins, which exhibit the characteristics including wide electrochemical working window, good biocompatibility, large surface area, high electrical conductivity, chemical and photochemical stability, ease of preparation and excellent substrate adhesion. Electrochemical impedance spectroscopy (EIS) was performed on a CHI 660D electrochemical workstation (Shanghai CH Instrument, China) in a 0.1 mol L–1 KCl solution containing 1.0 mmol L–1 [Fe(CN)6]3–/4– with the. NiO nanoflower was synthesized based on the reported procedure.[17] In brief 0.2 g of sulfonated polystyrene was dispersed into a solution containing 20 mL of deionized water and 20 mL of ethanol by sonication for 10 minutes. The resulted electrode was denoted as CTS/NiO-HRP/CILE and stored at 4 °C when not in use. Other modified electrodes such as CTS/HRP/CILE, CTS/NiO/CILE etc. were prepared by the similar procedures and used for the comparison
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