Abstract
AbstractA novel nanocomposite of colloidal gold (GNPs) and hydroxyapatite nanotubes (Hap) was prepared for immobilization of a redox protein, hemoglobin (Hb), on glassy carbon electrode. The immobilized Hb showed fast direct electron transfer and excellent electrocatalytic behavior toward reduction of hydrogen peroxide. A synergic effect between GNPs and Hap for accelerating the surface electron transfer of Hb was observed, which led to a pair of redox peaks with a formal potential of (−340±2) mV at pH 7.0, and a new biosensor for hydrogen peroxide with a linear range from 0.5 to 25 μM and a limit of detection of 0.2 μM at 3σ. Owing to the good biocompatibility of the nanocomposite, the biosensor exhibited good stability and acceptable reproducibility. The as‐prepared nanocomposite film provided a good matrix for protein immobilization and biosensor preparation.
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