Abstract
A well-defined amphiphilic diblock copolymer, poly [ N-(2-methacryloyloxyethyl) pyrrolidone]- block-poly [glycidyl methacrylate] (PNMP- b-PGMA), was applied to immobilize hemoglobin (Hb) in a simple procedure. The characterization of PNMP- b-PGMA/Hb film was demonstrated by ultraviolet–visible (UV–vis) spectra, atomic force microscopy (AFM), and electrochemical impedance spectroscopy (EIS). The electrochemical behaviors of Hb in PNMP- b-PGMA film have been investigated and a pair of well-defined and nearly reversible cyclic voltammetric peaks for the protein heme Fe(III)/Fe(II) redox couples were observed at about −0.409 V (vs. SCE) in pH 7.0 phosphate buffer solution (PBS). The results showed that the PNMP- b-PGMA film improved the protein loading with the retention of bioactivity and greatly promoted the direct electron transfer between Hb and electrode surface. This PNMP- b-PGMA/Hb film modified electrode also exhibited high electrocatalytic effects towards reduction of nitric oxide (NO). These results indicated the potential applicability of the films as new types of third-generation biosensors or bioreactors based on direct electrochemistry of the proteins.
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