Abstract
Cytochrome c/DNA modified electrode was achieved by coating calf thymus DNA onto the surface of glassy carbon electrode firstly, then immobilizing cytochrome c on it by multi-cyclic voltammetric method and characterized by the electrochemical impedance. The electrochemical behavior of cytochrome c on DNA modified electrode was explored and showed a quasi-reversible electrochemical redox behavior with a formal potential of 0.045 ± 0.010 V ( versus Ag/AgCl) in 0.10 M, pH 5.0, acetate buffer solution. The peak currents were linearly with the scan rate in the range of 20–200 mV/s. Cytochrome c/DNA modified electrode exhibited elegant catalytic activity for the electrochemical reduction of NO. The catalytic current is linear to the nitric oxide concentration in the range of 6.0 × 10 −7 to 8.0 × 10 −6 M and the detection limit was 1.0 × 10 −7 M (three times the ratio of signal to noise, S/N = 3).
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