Abstract
BackgroundCaffeine consumption has been reported to decrease bone mineral density (BMD), increase the risk of hip fracture, and negatively influence calcium retention. In this study, we investigated the influence of caffeine on the osteoblasts behaviour.MethodOsteoblasts derived from newborn Wistar-rat calvaria was used in this study. The effects of various concentrations of caffeine on bone cell activities were evaluated by using MTT assay. Alkaline phosphatase (ALP) staining, von Kossa staining and biochemical parameters including ALP, lactate dehydrogenase (LDH), prostaglandin E2 (PGE2) and total protein were performed at day 1, 3, and 7. DNA degradation analysis under the caffeine influence was also performed.Results and discussionThe results showed that the viability of the osteoblasts, the formation of ALP positive staining colonies and mineralization nodules formation in the osteoblasts cultures decreased significantly in the presence of 10 mM caffeine. The intracellular LDH, ALP and PGE2 content decreased significantly, the LDH and PGE2 secreted into the medium increased significantly. The activation of an irreversible commitment to cell death by caffeine was clearly demonstrated by DNA ladder staining.ConclusionIn summary, our results suggest that caffeine has potential deleterious effect on the osteoblasts viability, which may enhance the rate of osteoblasts apoptosis.
Highlights
Caffeine consumption has been reported to decrease bone mineral density (BMD), increase the risk of hip fracture, and negatively influence calcium retention
In summary, our results suggest that caffeine has potential deleterious effect on the osteoblasts viability, which may enhance the rate of osteoblasts apoptosis
When osteoblast cells cultured with caffeine for one day, there was no statistically significant change in the formation of formazan; while in the 100 mM to 1 mM concentration of caffeine, the formation of formazan was significantly decreased in the third day's culture (Fig. 1)
Summary
Caffeine consumption has been reported to decrease bone mineral density (BMD), increase the risk of hip fracture, and negatively influence calcium retention. We investigated the influence of caffeine on the osteoblasts behaviour. Caffeine and the related methyl xanthines are widely distributed in plants throughout the world. Caffeine-containing beverage consumption has been reported to be associated with reduced bone mass and increased fracture risk in some observational studies. Massey and colleagues [2] showed that a caffeine-induced diuresis increased urinary calcium loss acutely. Later controlled human physiological balance studies show a clear but only a very small depressant effect of caffeine on intestinal calcium absorption, and no effect on total 24-h urinary calcium excretion [3]
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