Abstract

Fourier transform infrared spectroscopy was used to characterise highland and lowland populations of Polygonum minus Huds. grown in different controlled environments. A thermal perturbation technique of two- dimensional correlation infrared spectroscopy (2D-IR) cor- relation spectra was applied to establish differences between the populations. The absorption peaks at 3,480 cm -1 (hydroxyl group), 2,927 cm -1 (methyl group), 1,623 cm -1 (carbonyl group), and 1,068 cm -1 (C-O group) were par- ticularly powerful in separating the populations. These peaks, which indicate the presence of carbohydrate, terp- enes, amide and flavonoids were more intense for the high- land populations than lowland populations, and increased in environments with a higher temperature. Wavenumbers (1,634, 669 cm -1 ) and (1,634, 1,555 cm -1 ) in the 2D-IR correlation spectra provided fingerprint signals to differen- tiate plants grown at different temperatures. This study demonstrates that IR fingerprinting, which combines mid-IR spectra and 2D-IR correlation spectra, can directly discrim- inate different populations of P. minus and the effects of temperature.

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