Direct diagnosis of Leishmania species on serosity materials punctured from cutaneous leishmaniasis patients using PCR-RFLP

Abstract

This study was aimed at identifying the Leishmania species using serosity materials punctured from skin lesions of cutaneous leishmaniasis (CL) patients by using internal transcribed spacer1 (ITS1) polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). We used the PCR-RFLP on 60 parasitological confirmed CL patients who referred to leishmaniasis laboratory from the School of Public Health, Tehran University of Medical Sciences. The PCR-RFLP could correctly detect 51 Leishmania species of the 60 confirmed positive specimens, where all the other 10 parasitological (microscopy and culture) negative samples that were prepared from other bacterial- and fungal-infected lesions had negative results. The results also revealed that Leishmania major was the dominant species (53.3%). This study suggests that the PCR-RFLP assay with serosity materials punctured from CL patients using Hae III enzyme is useful for the rapid identification of Leishmania species.