Abstract
1. 1. Dio 9, an antibiotic of unknown structure, and the synthetic bis-guanidine Chlorhexidine (1,6-di-4′-chlorophenyldiguanidohexane), inhibit the ATPase associated with isolated protoplast membranes of Streptococcus faecalis. They inhibit both the native, membrane-bound enzyme, and the solubilized protein. The effects of Dio 9 and Chlorhexidine differ in this respect from those of N,N′- dicyclohexylcarbodiimide which was previously found to inhibit only the membrane-bound ATPase. 2. 2. When added to intact cells, Dio 9 and Chlorhexidine inhibit the net uptake of K + by exchange for Na + and H +, and also the stimulation of glycolysis associated with K + uptake. However, they do not inhibit autologous exchange of 42K + for K +. 3. 3. Dio 9 and Chlorhexidine apparently do not inhibit the generation of ATP via glycolysis. Partial inhibition of glycolysis by these compounds may be a secondary consequence of the inhibition of ATPase. 4. 4. The finding that Dio 9 and Chlorhexidine inhibit both the ATPase and net K + uptake by S. faecalis supports the hypothesis that the ATPase is involved in ion transport.
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