Abstract
1. 1. The alimentary tract content has been divided into the stomach, pyloric caeca region, the middle intestine and the posterior intestine which was parted into two equal segments. 2. 2. Protein and free amino acids were analysed in digesta of subsequent parts of digestive tract in fish fed three different diets. 3. 3. An indirect method of apparent digestibility was employed to ascertain the absorption site of particular amino acids. 4. 4. A separate trial was performed to determine the metabolic amino acids excretion with the use of non-protein diet. Assuming that traces of amino acids supplied in non-protein diet should be subtracted from the metabolic ones, the metabolic amino acid excretion was calculated for subsequent parts of digestive tract. 5. 5. On the basis of the knowledge of metabolic amino acid excretion, the true digestibility of examined diets was calculated in the parts of digestive tract.
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