Abstract
Pleurotus ostreatus mycelium was grown in the presence of tobacco and wheat straw juices, ground wheat straw and acid-precipitable polymeric lignin with the aim of increasing the production of extracellular enzymes potentially implicated in lignin degradation. The highest production of diphenol oxidases was obtained when culture broths were supplemented with solid straw or tobacco juice; cellulase and veratryl alcohol oxidase were produced in detectable amounts in the presence of solid straw and in the absence of any inducer, respectively. No lignin peroxidase was found under any growth condition. Chromatographic fractionation of extracellular proteins and kinetic analysis of partially purified fractions showed that, in the absence of inducers, o-, p- and m-diphenol oxidases could be detected in column eluates, whereas all the inducers tested increased selectively the production of o-diphenol oxidases.
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