Abstract
Diabetes constitutes a worldwide epidemic affecting all ethnic groups. Stem cell therapy provides an effective way to regenerate insulin-producing cells lost during the disease. Accordingly, we isolated and compared the differentiation ability of nestin rich bone marrow mesenchymal stem cells (MSCs) into insulin producing cells (IPCs) with the nestin poor counterpart. The purity of the isolated populations was evaluated by immunofluorescence then, cells were differentiated into IPCs. At the end of differentiation, cells were evaluated by expression of pancreatic endocrine genes, immunofluorescence as well as the release of human insulin and c-peptide according to increasing glucose concentrations that showed a significant difference. Under confocal microscope the mean percentage of IPCs was 14.3% for nestin rich MSCs while nestin poor MSCs showed 2.17 %. In conclusion, nestin positive cells is a sub-population of MSCs, capable of generating IPCs in higher percentage than its negative counterpart. Key words: Nestin; Mesenchymal stem cells; differentiation; insulin producing cells.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
