Abstract
The rubber tree (Hevea brasiliensis Muell. Arg.) is a tropical tree species that produce natural rubber. Self-rooted juvenile clones (SRJCs) are novel rubber tree planting materials developed through primary somatic embryogenesis. SRJCs have a higher rubber yield compared with donor clones (DCs). The molecular basis underlying increased rubber yield in SRJCs remains largely unknown. Here, the latex from SRJCs and DCs were collected for strand-specific and small RNA-seq methods. A total of 196 differentially expressed long noncoding RNAs (DELs), and 11 differentially expressed microRNAs were identified in latex between SRJCs and DCs. Targeted genes of DELs were markedly enriched for various biological pathways related to plant hormone signal transduction, photosynthesis, glutathione metabolism, and amino acids biosynthesis. DELs probably acted as cis-acting regulation was calculated, and these DELs relevant to potentially regulate rubber biosynthesis, reactive oxygen species metabolism, and epigenetic modification. Furthermore, the DELs acting as microRNA targets were studied. The interaction of microRNA and DELs might involve in the regulation of natural rubber biosynthesis.
Highlights
Natural rubber, an important industrial raw material, is widely used to produce tires and medical gloves (Cornish, 2017)
The systematic identification of Long noncoding RNAs (lncRNAs) in rubber trees was performed in latex from Self-rooted juvenile clones (SRJCs) and donor clones (DCs) using strand-specific RNA (ssRNA)-seq
Among these lncRNA–mRNA pairs, XR_002492989.1 was spaced 4.26 kb upstream of XM_021798588.1, which encoded a 3-hydroxy-3methylglutaryl-coenzyme A reductase (HMGR) that catalyzes the conversion of HMG-CoA to mevalonic acid, which is a central step for the biosynthesis of a number of natural products (Figure 5A)
Summary
An important industrial raw material, is widely used to produce tires and medical gloves (Cornish, 2017). Natural rubber production is derived entirely from the rubber tree Arg.; Cherian et al, 2019). The rubber tree is a type of crosspollinating plant. High-yielding varieties of rubber trees are bred through cross programs. The rubber tree is propagated by grafting axillary buds onto rootstocks grown from cross-pollinated seeds to preserve the desirable characters of an elite variety (Chandrashekar et al, 1997). Given the interactions between rootstock and scion, budding clones often show remarkable intraclonal variations in growth potential and rubber yield (Clément-Demange et al, 2007)
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