Differential efficiency of photosynthetic oxygen evolution in flashing light in triazine-resistant and triazine-susceptible biotypes of Senecio vulgaris L.

Abstract

Photosynthetic oxygen evolution in response to flashing light was studied in triazine-susceptible and triazine-resistant biotypes of Senecio vulgaris L. Studies were conducted to determine if the modification of the herbicide-binding site which confers s-triazine resistance also affects the oxygen-evolving system. Oxygen evolution was measured using a Joliot-type oxygen-specific electrode on broken, stroma-free chloroplasts of both biotypes. We observed abnormal patterns of oxygen evolution in resistant chloroplasts. The S′ 1 → S 2 transition is slower while the S 2 decay is faster. The S′ 2 → S 3 transition, in contrast, is slightly faster in resistant chloroplasts, while the decay of the S 3 state is the same as in susceptible chloroplasts. These altered kinetics may be due to altered Q → B (B −) electron flow in resistant chloroplasts. These results are also consistent with the hypothesis that back-reactions from the reducing (acceptor) side of Photosystem II to the oxidizing (donor) side occur with greater frequency in resistant than susceptible chloroplasts. These events are responsible for lower oxygen yield and increased ‘misses’ and ‘double hits,’ resulting in abnormal yield patterns and lower quantum yield of CO 2 fixation in resistant chloroplasts compared to the susceptible ones.