Differential effects of actinomycin D and puromycin on the release of interferon induced by double stranded RNA.

Abstract

THE induction of interferon production with synthetic double stranded RNA1 is suppressed by actinomycin D2,3. Finkelstein et al.4, however, reported that considerably higher concentrations of actinomycin D were required for the suppression of interferon production induced by double stranded RNA than for the inhibition of viral-induced interferon synthesis. Moreover, the release of interferon after treatment with double stranded RNA in the intact organism5 or in cell cultures4 was not suppressed by inhibitors of protein synthesis, while viral-induced interferon synthesis was inhibited in the same conditions.