Abstract
BackgroundExosomal circular RNAs (circRNAs) are emerging as important regulators of physiological development and disease pathogenesis. However, the roles of exosomal circRNAs from umbilical cord blood in preeclampsia (PE) occurrence remains poorly understood.MethodsWe used microarray technology to establish the differential circRNA expression profiles in umbilical cord blood exosomes from PE patients compared with normal controls. Bioinformatics analysis was conducted to further predict the potential effects of the differentially expressed circRNAs and their interactions with miRNAs.ResultsAccording to the microarray data, we identified 143 significantly up-regulated circRNAs and 161 significantly down-regulated circRNAs in umbilical cord blood exosomes of PE patients compared with controls. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analyses showed that circRNA parental genes involved in the regulation of metabolic process, trophoblast growth and invasion were significantly enriched, which play important roles in PE development. Moreover, pathway network was constructed to reveal the key pathways in PE, such as PI3K-Akt signaling pathway. Further circRNA/miRNA interactions analysis demonstrated that most exosomal circRNAs had miRNA binding sites, and some miRNAs were associated with PE.ConclusionsOur results highlight the importance of exosomal circRNAs in the pathogenesis of PE and lay a foundation for extensive studies on the role of exosomal circRNAs in PE development.
Highlights
Exosomal circular RNAs are emerging as important regulators of physiological development and disease pathogenesis
We found that the levels of Circular RNA (circRNA) expression in umbilical cord blood exosomes were distinguishable between PE patients and the control group (Fig. 1a)
The results showed that the expression of circRNAs in cord blood exosomes was different between PE patients and the control group
Summary
Exosomal circular RNAs (circRNAs) are emerging as important regulators of physiological development and disease pathogenesis. Preeclampsia (PE) is a gestation-specific syndrome that affects up to 5–7% of pregnancies, characterized by elevated blood pressure and proteinuria after 20 weeks of pregnancy [1, 2]. This multisystem pregnancy disorder is often accompanied by headache, nausea, vomiting, upper abdominal discomfort and other symptoms, and is a leading cause of maternal and neonatal morbidity and mortality worldwide [3]. We speculated that exosomal circRNA in umbilical cord blood might play an important role in the regulation of PE development as a new placental derived factor. We used microarray technology to construct a comparative exosomal circRNA profiling of umbilical cord blood between PE patients and controls, aiming to lay a foundation for further research on the role of exosomal circRNAs in PE development
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