Abstract
Summary Changes in seedling growth and Glutamine Synthetase isoforms in cotyledons of germinating chickpea ( Cicer arietinum L.) seeds were studied in response to osmotic stress. PEG-induced osmotic stress adversely affected the seedling growth. After 6h of imbibition, only one GS isoform was detected in the cotyledons while at all subsequent stages both cytosolic (GS 1 ) and plastidic (GS 2 ) isoforms were observed. GS 1 constituted a major proportion of total GS activity in the cotyledons of control-germinated seeds but under osmotic stress GS 1 activity decreased, whereas GS 2 activity increased. Implications of these findings in stress adaptation of germinating chickpea seeds are discussed.
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