Differential accumulation and localization of maternal poly(A)-containing RNA during early development of the ascidian, Styela

Abstract

The regional distribution of poly(A) + RNA was examined in sections of Styela oocytes and fertilized eggs by in situ hybridization with [ 3H]poly(U). The nucleus and cytoplasm of previtellogenic oocytes contain equivalent densities of [ 3H]poly(U) binding sites. The concentration of these sites is reduced in the cytoplasm, but not the nucleus, during vitellogenesis. Consequently, the germinal vesicle (GV) plasm of mature oocytes is characterized by an eightfold elevation in [ 3H]poly(U) binding activity relative to the surrounding cytoplasm. The distinctive cytoplasmic regions of the mature oocyte do not exhibit differential concentrations of [ 3H]poly(U) binding sites. Following fertilization which triggers GV breakdown, meiosis, and ooplasmic segregation, the high density of [ 3H]poly(U) binding sites characteristic of the GV plasm is conserved in the basophilic cytoplasm during its extensive migration and eventual accumulation in the animal hemisphere of the egg. The insensitivity of the [ 3H]poly(U) binding sites of the basophilic cytoplasm to actinomycin D suggests that they are of maternal origin. It is concluded that maternal poly(A) + RNA is subject to differential accumulation in the GV plasm and its derivative ooplasm during the early development of Styela.