Different strategies of grass carp (Ctenopharyngodon idella) responding to insufficient or excessive dietary carbohydrate

Abstract

As typical herbivorous fish, grass carp possesses greater capability to utilize carbohydrate than carnivorous and omnivorous fish. However, mechanism of carbohydrate utilization in herbivorous fish was unclear. This study is intended to investigate response of grass carp to insufficient or excessive dietary carbohydrate. Grass carp accepted isonitrogenous diets with different carbohydrate levels (15.90, 21.81 and 45.45 g 100 g−1) were cultured. After 8 weeks, weight gain and feed intake were highest in fish fed with medium-carbohydrate diet compared with others. Gene expression data indicated that expressions of glucose-6-phosphatase (G6Pase) were down-regulated and gene expressions of glycogen synthase (GS) were up-regulated in fish accepted lower-carbohydrate diet. In high-carbohydrate group, glucose catabolism was increased by enhanced expressions of glucokinase genes (GK). Meanwhile, fatty acid synthase (FAS) and acetyl coenzyme-A carboxylase (ACC1) genes were significantly up-regulated and lipoprotein lipase and carnitine palmitoyltransferase 1 (CPT1) genes expressions were down-regulate, inducing more lipid deposition. Subsequently, serum cholesterol and total lipid contents were significantly increased, and then induced appetite suppression through up-regulation of leptin and anorexigenic genes expressions. Furthermore, depressed food intake caused lower growth rate in grass carp with excessive carbohydrate intake.