Abstract
Methods Monocytes from 6 CINCA and 4 TRAPS patients selected for treatment with Anakinra were activated with 1 μg/ml of LPS for 3 hours, at baseline and after 7 days from the beginning of the treatment. For comparison, monocytes from 24 healthy donors were also studied. Intracellular pro-IL-1β and secreted IL-1β were analysed by Western blotting and ELISA before and after a short exposure (15 min) to exogenous ATP that accelerates IL-1β secretion.
Highlights
D Lasigliè*1, S Carta2, S Tassi2, F Ferlito1, A Piccini2, A Martini1, A Rubartelli2 and M Gattorno1
Despite a similar clinical response to anti-IL1 treatment, the pattern of IL-1β secretion of monocytes from Anakinra-responder TRAPS patients significantly differ from that observed in patients CIAS-1 mutations
This study suggests a different hierarchy in the pathogenic mechanisms leading to the inflammatory response in different diseases responsive to anti-IL-1 treatment
Summary
D Lasigliè*1, S Carta, S Tassi, F Ferlito, A Piccini, A Martini, A Rubartelli and M Gattorno. Gaslini" Institute and Department of Pediatrics University of Genoa, Genoa, Italy and 2Laboratory of Experimental Oncology E/Cell Biology, Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy. Published: 15 September 2008 Pediatric Rheumatology 2008, 6(Suppl 1):P212 doi:10.1186/1546-0096-6-S1-P212. 15th Paediatric Rheumatology European Society (PreS) Congress Wietse Kuis, Patricia Woo, Angelo Ravelli, Hermann Girschick, Michaël Hofer, Johannes Roth, Rotraud K Saurenmann, Alberto Martini, Pavla Dolezova, Janjaap van der Net, Pierre Quartier, Lucy Wedderburn and Jan Scott Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
