Different genotypes of Silene vulgaris (Moench) Garcke grown on chromium-contaminated soils influence root organic acid composition and rhizosphere bacterial communities.

Abstract

Plant-microbe interactions are considered to be important processes determining the efficiency of phytoremediation of heavy metal-contaminated soils. However, relatively little is known about how these interactions are influenced by chromium (Cr) contamination. The effect of Cr stress on metal uptake, root organic acid composition, and rhizosphere bacterial communities was studied using two genotypes of the metallophyte Silene vulgaris, which have shown different tolerance to Cr(VI). The results indicated that root biomass and shoot biomass were not significantly influenced by Cr treatment, but metal uptake in shoots and roots was significantly impacted by the genotype. Principal component analyses (PCA) showed that variation in organic acids oxalic, citric, malic, formic, lactic, acetic, and succinic differed between genotypes. Changes in root organic acid contents in response to Cr revealed a significant increase of oxalic acid in genotype SV-21. The denaturing gradient gel electrophoresis (DGGE) cluster analysis showed that the community structure (determined by PCR-DGGE) was affected by plant genotype and, to a lesser extent, by Cr contamination, the first being the most influential factor shaping the rhizosphere microbiome. Under Cr pollution, a shift in the relative abundance of specific taxa was found and dominant phylotypes were identified as Variovorax in SV-21 and Chitinophaga niastensis, Pontibacter sp., and Ramlibacter sp. in SV-38. These results provided the basis for further studies aimed at the combined use of plants and soil microorganisms in the remediation of Cr-polluted soils.