Abstract
The sensitivity in vitro against gamma-radiation of valyl-tRNA synthetase (VRS) from chick embryo brain proved to be higher than that of liver VRS. In order to study this phenomenon properties of VRS isolated from both organs were compared. Both enzymes have the same molecular weight (Mr = 110 000) determined by equilibrium sedimentation and by gel filtration. Electrophoresis on polyacrylamide gel in the presence of sodium dodecyl sulphate showed no evidence for subunit structure. The optimal reaction conditions in valyl-tRNA formation were found to be the same, except the pH values. Km values of the reactions were also similar. Both enzymes reacted with the tRNAs isolated from the other organ. The stability during storage of the liver VRS was higher than that of the brain VRS. In the brain VRS molecule one buried and eleven free SH groups could be detected and divided into three classes with different reactivities. In 1 mol of liver VRS one buried and nine free thiol groups were determined and these could be divided only into two classes. These observations suggest the existence of two VRS isoenzymes with different radiosensitivities: the more sensitive occurs mainly in brain and the less sensitive mainly in the liver of chick embryo.
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