Abstract
The authors investigated the dielectric behavior of mammalian DNA macromolecules of different sizes in aqueous solution, by dielectric relaxation spectroscopy in the frequency range 100 Hz to 1 MHz, during the double helix to single coil thermal transition, in association with the gradual disruption of the hydrogen bonds between the complementary nitrogenous bases of the DNA double helix, measured by thermal transition spectrophotometry. The authors' results have shown that, when the temperature of DNA samples is gradually increasing, changes in specific conductivity /spl sigma//sub dc/ precede changes in A/sub 260/ absorbance at the UV region, where A/sub 260/ is related to the disruption of the hydrogen bonds between the two DNA strands. The presented experimental evidence extends the validation of multistage models for this transition phase to the case of large size mammalian native macromolecular DNA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: IEEE Transactions on Dielectrics and Electrical Insulation
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
