Abstract

The article reflects the results of testing various diagnostic tests (ST, RA, CFT, BRT, IHA and ELISA) in the diagnosis of brucellosis in cattle, where animals are subjected to serological tests without the use of a vaccine. Antibodies against brucellosis were detected in diagnostic titer in ST in 100% and in ELISA in 96% of cases. The difference between the rates of these reactions was statistically insignificant (P> 0.1). ICA is significantly inferior to these two tests. This difference turned out to be significant, that is, significant (P <0.01). The lowest rates are set for RA, RBT and CFT. For the diagnosis of brucellosis, of all the tests tested, ST and ELISA turned out to be the most sensitive, and of these two tests, the most accessible for practice is ST.

Highlights

  • Bovine brucellosis is a widespread zoonotic disease in many countries of the world that causes great economic damage to livestock and poses a serious threat to human health [1,2,3]

  • The largest number of studies has been devoted to the study of the diagnostic value of the agglutination reaction (RA), the complement fixation reaction (CFT) and its long-term variant (LTV), the Bengal rose test (BRT)

  • Anti-brucellosis antibodies were found in diagnostic titers for Saiduldin Test (ST) in 100%, and in enzyme-linked immunosorbent assay (ELISA) in 96% of cases

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Summary

Introduction

Bovine brucellosis is a widespread zoonotic disease in many countries of the world that causes great economic damage to livestock and poses a serious threat to human health [1,2,3]. One of the main methods of rehabilitation of unsuccessful foci of brucellosis in cattle in our republic and some countries of the CIS and far abroad is the systematic conduct of serological studies followed by the slaughter of reacting animals and appropriate sanitary measures [1, 2, 4]. On one farm, during 136-154 days, they were examined 6 times (every 15-30 days), as a result of which the diagnosis of brucellosis was confirmed in 91.3% of cows and 83% of heifers On another farm, 70% of the isolated animals were diagnosed within 90 days. Testing of the diagnostic values of serological methods (ST, AT, RBT, CFT, ICA and ELISA) in the diagnosis of brucellosis of cattle in farms and settlements unfavorable for this disease, where animals have not been subjected to antibrucellosis vaccinations

Materials and methods
Research results and discussion
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