Abstract
This study was aimed to identify and confirm of E.coli isolated from arthritis in chickens. Samples for the isolation of the bacteria were taken from broiler chickens with arthritis symptoms (swelling hock joint), and then examined by culturing, VITEK test as well as molecular assay. Antimicrobial susceptibility of bacterial isolate was done. The results showed pink colour colonies on MacConkey agar. The VITEK system was used to identify those colonies. The results revealed that the isolate gave 97 % parallel to those features of E. coli equally recognized by the standard gram negative card. The isolate was found to be sensitive to ticarcillin/clavulanic acid and many others antibiotics and resistance to ticarcillin as well as for other types of antibiotics .The results of the 16Sr RNA gene revealed that E.coli primers of the16S rRNA gene had successfully targeted the respective gene and have shown the single bands of the16S RNA gene at 1500 bp. Sequencing of this gene was performed for the isolate. The results of nucleotide sequencing were submitted in Gene bank database and have accession number: ID: MT012194.1. The phylogenetic analysis Of the isolate was 100% similar to USA:CP048605, Pakistan:GU594300, VitNamHatey: AP022650 and China:MN208222, however, it was 99% similar to India:LCO58573.
Highlights
Escherichia coli (E. coli) is often responsible for flock outbreaks of arthritis and osteomyelitis in chickens which are sequelae of septicemia, and these outbreaks may be associated with enteric or respiratory disease[8]
There is no information about molecular features of "E. coli strains isolated from lesions in the locomotor system of broilers" in Iraq, the aim of this study was planned to identify E.coli isolated from arthritis in broiler chickens by VITEK system and molecular assay
A loopful of the culture suspension was streaked onto MacConkey agar and incubated at 37oC for [24-48] hrs. following a day, those pinkish colonies most likely an E. coli were subcultured onto nutrient agar to acquire a pure colony, followed by subculture, putative E. coli colonies were further identification was done by VITEK testing by means of commercially obtainable proof of identity cards for gram-negative bacteria "in accordance to the manufacturer’s recommendations “ examined antibiotic susceptibility by VITEK card [9]
Summary
Escherichia coli (E. coli) is often responsible for flock outbreaks of arthritis and osteomyelitis in chickens which are sequelae of septicemia, and these outbreaks may be associated with enteric or respiratory disease[8]. تم إج ارء هذه الد ارسة لعزل وتوصيف ايشريكيا القولون من دجاج مصاب بالتهاب المفاصل وتم اخذ عينات للعزل البكتيري تم
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
