Abstract
During pregnancy, diabetes insipidus can arise following the increase in serum of vasopressinase activity, which is identical to oxytocinase/insulin-regulated membrane aminopeptidase (Otase/IRAP) or cystine-aminopeptidase/insulin-regulated membrane aminopeptidase (CAP/IRAP) or EC. 3.4.11.3. First, we measured the vasopressinase activity in 1445 sera from normal pregnant women using S-benzyl-L-cystéine-4-nitroanilide as substrate. Then, in opposition to results previously published with the use of L-leucine- p-nitroanilide we evidenced that 20 mM L-methionine did not permit to differentiate placental and non-placental activity when S-benzyl-L-cystéine-4-nitroanilide was used as substrate. Finally, the S-benzyl-L-cystéine-4-nitroanilide, when used without addition of L-methionine in the incubation mixtures, appears to be a more suitable substrate for measuring vasopressinase than L-leucine- p-nitroanilide.
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