Developmental shift of short-term synaptic plasticity in cortical organotypic slices

Abstract

Although short-term synaptic plasticity (STP) is ubiquitous in neocortical synapses its functional role in neural computations is not well understood. Critical to elucidating the function of STP will be to understand how STP itself changes with development and experience. Previous studies have reported developmental changes in STP using acute slices. It is not clear, however, to what extent the changes in STP are a function of local ontogenetic programs or the result of the many different sensory and experience-dependent changes that accompany development in vivo. To address this question we examined the in vitro development of STP in organotypic slices cultured for up to 4weeks. Paired recordings were performed in L5 pyramidal neurons at different stages of in vitro development. We observed a shift in STP in the form of a decrease in the paired-pulse ratio (PPR) (less depression) from the second to fourth week in vitro. This shift in STP was not accompanied by a change in initial excitatory postsynaptic potential (EPSP) amplitude. Fitting STP to a quantitative model indicated that the developmental shift is consistent with presynaptic changes. Importantly, despite the change in the PPR we did not observe changes in the time constant governing STP. Since these experiments were conducted in vitro our results indicate that the shift in STP does not depend on in vivo sensory experience. Although sensory experience may shape STP, we suggest that developmental shifts in STP are at least in part ontogenetically determined.