Abstract
In this study, we have compared the skeletal muscle proteome at various stages of porcine postnatal development. Korean native pigs were divided into five postnatal stages of 30, 70, 130, 170 and 300 d and their loin muscles were analyzed for muscle proteome by using two-dimensional electrophoresis and mass spectrometry. We found 5 proteins showing a consistent pattern during skeletal muscle growth. Four proteins were identified as myosin light chain 1 slow-twitch (MLC1sa) isoform, troponin T, triosephosphate isomerase (TIP) and DJ-1 protein. The remaining protein was not identified. Two muscle fiber proteins of MLC1sa isoform and troponin T showed a high expression level at an early postnatal stage and then their levels were decreased markedly during growth stages. On the other hand, the expression of TIP and DJ-1 protein, which are well known as catalysis enzyme and antioxidant-related protein, respectively, were linearly increased during growth stages. Thus, the stage-related muscle proteins may be useful as parameters for understanding the developmental characteristics of biochemical and physiological properties in Korean native pig skeletal muscle.
Highlights
In this study, we have compared the skeletal muscle proteome at various stages of porcine postnatal development
The key elements of classical proteomics are the separation of proteins in a sample using two-dimensional gel electrophoresis (2-DE) and their subsequent quantitation and identification
For comparing the developmental changes of muscle proteome levels, Korean native black pigs were divided into five postnatal stages (30, 70, 130, 170 and 300 d) and their loin muscle proteomes were analyzed by 2-DE and mass spectrometry
Summary
We have compared the skeletal muscle proteome at various stages of porcine postnatal development. Korean native pigs were divided into five postnatal stages of 30, 70, 130, 170 and 300 d and their loin muscles were analyzed for muscle proteome by using two-dimensional electrophoresis and mass spectrometry. We found 5 proteins showing a consistent pattern during skeletal muscle growth. Two muscle fiber proteins of MLC1sa isoform and troponin T showed a high expression level at an early postnatal stage and their levels were decreased markedly during growth stages. The stage-related muscle proteins may be useful as parameters for understanding the developmental characteristics of biochemical and physiological properties in Korean native pig skeletal muscle. Proportion of the whole body results in a 30-fold increase in the changes of skeletal muscle proteome on growth muscle protein with only a 13-fold increase in body weight have not been previously investigated
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